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Oxford Nanopore exp-nbd104/114 native barcoding expansion kits
Exp Nbd104/114 Native Barcoding Expansion Kits, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/native+barcoding+expansion+kit+exp-nbd104/rapid+barcoding+kit+sqk+rbk004/pm40649886-180-25-30
Average 90 stars, based on 1 article reviews
exp-nbd104/114 native barcoding expansion kits - by Bioz Stars, 2026-10
90/100 stars

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Ligation:

Article Title: Culture and amplification-free nanopore sequencing for rapid detection of pathogens and antimicrobial resistance genes from urine
Article Snippet: .. The library was made using the Ligation Sequencing Kit (SQK-LSK109) and Native Barcoding Expansion Kit (EXP-NBD104) according to the manufacturer’s instructions and sequenced using Flongle flow cells (R9.4.1 FLO-FLG001, Oxford Nanopore). ..

Article Title: AAV2 can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virus
Article Snippet: Following RCA, samples were digested with Hin dIII (R0104S, New England Biolabs, MA, USA) or left untreated prior to sequencing or Southern blotting. .. Extrachromosomal DNA was extracted from infected cells using the Hirt protocol ( ) and prepared for Oxford Nanopore sequencing using the ligation sequencing kit (LSK109, Oxford Nanopore Technologies, Oxford, UK) and the native barcoding expansion kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) according to the manufacturers’ protocols. .. The samples were sequenced using a MinION sequencing device and flow cell (FLO-MIN_106, Oxford Nanopore Technologies, Oxford, UK).

Article Title: Emergence of multidrug-resistant E. coli ST8346 isolates carrying three distinct plasmids with NDM-5, KPC-2, and OXA-181.
Article Snippet: The long-read sequencing libraries were constructed using the KAPA HyperPlus Kit (KAPA Biosystems, Roche, Pleasanton, USA), which involves steps such as end repair, A-tailing, barcoding for sample multiplexing, and adapter ligation. .. Barcode ligation was performed with the Native Barcoding Expansion Kit (EXP-NBD104, ONT, New York, USA), and adapter ligation was performed with the Oxford Nanopore Technologies (ONT) Ligation Library Preparation Kit (SQK-LSK109, ONT, New York, USA). .. DNA libraries were purified with KAPA Hyper Beads (KAPA Biosystems, Roche, Pleasanton, USA) at a 0.4x ratio to enrich DNA fragments > 3 kb.

Article Title: Carriage Rate of Enterobacterales Resistant to Extended-Spectrum Cephalosporins in the Tunisian Population
Article Snippet: .. The MinION long-read sequencing libraries were prepared following Oxford Nanopore MinION Technologies (Oxford, UK) protocols, using the native barcoding expansion kit (EXP-NBD104) and the ligation sequencing kit (SQK-LSK109). .. Sequencing was carried out on a MinION device equipped with a SpotON Mk 1 R9 version flow cell (FLO-MIN106D).

Article Title: AAV2 can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virus.
Article Snippet: Following RCA, samples were digested with HindIII (R0104S, New England Biolabs, MA, USA) or left untreated prior to sequencing or Southern blotting. .. Extrachromosomal DNA was extracted from infected cells using the Hirt protocol (39) and prepared for Oxford Nanopore sequencing using the ligation sequencing kit (LSK109, Oxford Nanopore Technologies, Oxford, UK) and the native barcoding expansion kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) according to the manufacturers’ protocols. .. The samples were sequenced using a MinION sequencing device and flow cell (FLO-MIN_106, Oxford Nanopore Technologies, Oxford, UK).

Sequencing:

Article Title: Culture and amplification-free nanopore sequencing for rapid detection of pathogens and antimicrobial resistance genes from urine
Article Snippet: .. The library was made using the Ligation Sequencing Kit (SQK-LSK109) and Native Barcoding Expansion Kit (EXP-NBD104) according to the manufacturer’s instructions and sequenced using Flongle flow cells (R9.4.1 FLO-FLG001, Oxford Nanopore). ..

Article Title: AAV2 can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virus
Article Snippet: Following RCA, samples were digested with Hin dIII (R0104S, New England Biolabs, MA, USA) or left untreated prior to sequencing or Southern blotting. .. Extrachromosomal DNA was extracted from infected cells using the Hirt protocol ( ) and prepared for Oxford Nanopore sequencing using the ligation sequencing kit (LSK109, Oxford Nanopore Technologies, Oxford, UK) and the native barcoding expansion kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) according to the manufacturers’ protocols. .. The samples were sequenced using a MinION sequencing device and flow cell (FLO-MIN_106, Oxford Nanopore Technologies, Oxford, UK).

Article Title: Carriage Rate of Enterobacterales Resistant to Extended-Spectrum Cephalosporins in the Tunisian Population
Article Snippet: .. The MinION long-read sequencing libraries were prepared following Oxford Nanopore MinION Technologies (Oxford, UK) protocols, using the native barcoding expansion kit (EXP-NBD104) and the ligation sequencing kit (SQK-LSK109). .. Sequencing was carried out on a MinION device equipped with a SpotON Mk 1 R9 version flow cell (FLO-MIN106D).

Article Title: AAV2 can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virus.
Article Snippet: Following RCA, samples were digested with HindIII (R0104S, New England Biolabs, MA, USA) or left untreated prior to sequencing or Southern blotting. .. Extrachromosomal DNA was extracted from infected cells using the Hirt protocol (39) and prepared for Oxford Nanopore sequencing using the ligation sequencing kit (LSK109, Oxford Nanopore Technologies, Oxford, UK) and the native barcoding expansion kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) according to the manufacturers’ protocols. .. The samples were sequenced using a MinION sequencing device and flow cell (FLO-MIN_106, Oxford Nanopore Technologies, Oxford, UK).

Infection:

Article Title: AAV2 can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virus
Article Snippet: Following RCA, samples were digested with Hin dIII (R0104S, New England Biolabs, MA, USA) or left untreated prior to sequencing or Southern blotting. .. Extrachromosomal DNA was extracted from infected cells using the Hirt protocol ( ) and prepared for Oxford Nanopore sequencing using the ligation sequencing kit (LSK109, Oxford Nanopore Technologies, Oxford, UK) and the native barcoding expansion kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) according to the manufacturers’ protocols. .. The samples were sequenced using a MinION sequencing device and flow cell (FLO-MIN_106, Oxford Nanopore Technologies, Oxford, UK).

Article Title: AAV2 can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virus.
Article Snippet: Following RCA, samples were digested with HindIII (R0104S, New England Biolabs, MA, USA) or left untreated prior to sequencing or Southern blotting. .. Extrachromosomal DNA was extracted from infected cells using the Hirt protocol (39) and prepared for Oxford Nanopore sequencing using the ligation sequencing kit (LSK109, Oxford Nanopore Technologies, Oxford, UK) and the native barcoding expansion kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) according to the manufacturers’ protocols. .. The samples were sequenced using a MinION sequencing device and flow cell (FLO-MIN_106, Oxford Nanopore Technologies, Oxford, UK).

Adapter Ligation:

Article Title: Emergence of multidrug-resistant E. coli ST8346 isolates carrying three distinct plasmids with NDM-5, KPC-2, and OXA-181.
Article Snippet: The long-read sequencing libraries were constructed using the KAPA HyperPlus Kit (KAPA Biosystems, Roche, Pleasanton, USA), which involves steps such as end repair, A-tailing, barcoding for sample multiplexing, and adapter ligation. .. Barcode ligation was performed with the Native Barcoding Expansion Kit (EXP-NBD104, ONT, New York, USA), and adapter ligation was performed with the Oxford Nanopore Technologies (ONT) Ligation Library Preparation Kit (SQK-LSK109, ONT, New York, USA). .. DNA libraries were purified with KAPA Hyper Beads (KAPA Biosystems, Roche, Pleasanton, USA) at a 0.4x ratio to enrich DNA fragments > 3 kb.

Multiplexing:

Article Title: Toxigenic Clostridium perfringens Isolated from At-Risk Paediatric Inflammatory Bowel Disease Patients
Article Snippet: For library preparation, the Ligation Sequencing Kit [Oxford Nanopore Technologies, SQK-LSK109] was used with an input of 1 μg of genomic DNA. .. Sample multiplexing was performed using 500 ng of end-repaired DNA with the Native Barcoding Expansion Kit [Oxford Nanopore Technologies, EXP-NBD104]. .. For Illumina library preparation, the Nextera DNA Flex kit [Illumina] was used with an input of 100 ng of genomic DNA.

other:

Article Title: Morphogenesis, starvation, and light responses in a mushroom-forming fungus revealed by long-read sequencing and extensive expression profiling.
Article Snippet: The proportionally mixed barcoded samples were used to prepare libraries with the Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore Technologies), which were then sequenced on two MinION flow cells (FLO-MIN106 R9.4.1, Oxford Nanopore Technologies).



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